Acropora Larvae Dna Rna Extraction Test Results

Larvae from FACE PUF Morea Project


For detailed extraction protocol view post: Protocol for DNA/RNA Extractions of Montipora Coral Larvae Using Zymo-Duet Extraction Kit


Extraction Date: May 17, 2019

Samples

ID Spawning Date Content Sample Quantity (µL) Sample Conserv. Tech
120 2/11/2018 Eggs 100 300 uL Shield Erin
797 4/11/2018 Eggs 100 300 uL Shield Erin

Extraction Notes

  • Samples were already preserved in 300µL DNA/RNA Sheild so did not add more
  • Digested for 3 hours and 40 minutes at 55°C at speed 35 in Incubator-Genie because test tubes were too large for the thermomixer
  • Added additional 15µL Proteinase K after 2 hours and 50 min
  • Some lipids left in samples
  • Pelleted at 2300 rcf for 1 min

Qubit
Quantified May 22, 2019

  • Followed Broad Range dsDNA and RNA Qubit protocol
  • All samples were read twice
Extraction # Sample DNA Standard 1 (RFU) DNA Standard 2 (RFU) DNA 1 (ng/µl) DNA 2 (ng/µl) Average DNA RNA Standard 1 (RFU) RNA Standard 2 (RFU) RNA 1 (ng/µl) RNA 2 (ng/ul) Average RNA
73 120 184.2 20357 Too Low Too Low Too Low 385.6 10602 60.4 60.4 60.4
74 797 184.2 20357 Too Low Too Low Too Low 385.6 10602 88.2 88.0 88.1

Tape Station

TS-biomin-Ext-Batch-12-73.png TS-biomin-Ext-Batch-12-74.png

Written on May 22, 2019