Larvae from Biomineralization Project

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For detailed extraction protocol view post: Protocol for DNA/RNA Extractions of Montipora Coral Larvae Using Zymo-Duet Extraction Kit

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Extraction Date: May 6, 2019

Samples

Extraction #	Sample #	Date Collected	Quantity (µL)	Extracter
52	127	6/13/2018	300	Erin
53	129	6/13/2018	300	Erin
54	132	6/13/2018	300	Erin
55	162	6/14/2018	300	Erin
56	167	6/14/2018	300	Erin
57	168	6/14/2018	300	Erin
58	179	6/14/2018	300	Erin
59	185	6/14/2018	300	Erin

Extraction Notes

• Gathered all samples and set aside on ice. Then added DNA/RNA Shield to all.
• Digested for 3 hours and 5 minutes at 55°C

Qubit
Quantified on May 10, 2019

• Followed Broad Range dsDNA and RNA Qubit protocol
• All samples were read twice

Extraction #	Sample	DNA Standard 1 (RFU)	DNA Standard 2 (RFU)	DNA 1 (ng/µl)	DNA 2 (ng/µl)	Average DNA	RNA Standard 1 (RFU)	RNA Standard 2 (RFU)	RNA 1 (ng/µl)	RNA 2 (ng/ul)	Average RNA
52	127	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	33.2	32.6	32.9
53	129	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	38.6	38.4	38.5
54	132	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	20.2	20.2	20.2
55	162	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	47.2	47.0	47.1
56	167	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	29.0	28.8	28.9
57	168	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	23.6	23.6	23.6
58	179	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	28.4	28.2	28.3
59	185	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	36.4	36.8	36.6

Tape Station

• Followed RNA TapeStation protocol

Tape stationed May 22, 2019