Larvae from Biomineralization Project

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For detailed extraction protocol view post: Protocol for DNA/RNA Extractions of Montipora Coral Larvae Using Zymo-Duet Extraction Kit

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Extraction Date: May 7, 2019

Samples

Extraction #	Sample #	Date Collected	Quantity (µL)	Extracter
60	128	6/13/2018	300	Erin and Emma
61	131	6/13/2018	300	Erin and Emma
62	134	6/13/2018	300	Erin and Emma
63	157	6/13/2018	300	Erin and Emma
64	159	6/13/2018	300	Erin and Emma
65	180	6/14/2018	300	Erin and Emma

Extraction Notes

• Gathered all samples and set aside on ice. Then added DNA/RNA Shield to all.
• Digested for 2 hours and 5 minutes at 55°C
• Some lipids left in samples
• Pelleted at 21130 rcf for 1 min

Qubit
Quantified on May 10, 2019

• Followed Broad Range dsDNA and RNA Qubit protocol
• All samples were read twice

Extraction #	Sample	DNA Standard 1 (RFU)	DNA Standard 2 (RFU)	DNA 1 (ng/µl)	DNA 2 (ng/µl)	Average DNA	RNA Standard 1 (RFU)	RNA Standard 2 (RFU)	RNA 1 (ng/µl)	RNA 2 (ng/ul)	Average RNA
60	128	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	158	158	158
61	131	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	131	132	131.5
62	134	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	118	118	118
63	157	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	75.6	76.6	76.1
64	159	143.3	18426	Too Low	Too Low	Too Low	389.4	11715	212	212	212
65	180	143.3	18426	3.20	3.12	3.16	389.4	11715	103	107	105

Tape Station

• Followed RNA TapeStation protocol