Larvae from Biomineralization Project

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For detailed extraction protocol view post: Protocol for DNA/RNA Extractions of Montipora Coral Larvae Using Zymo-Duet Extraction Kit

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Extraction Date: May 17, 2019

Samples

Extraction #	Sample #	Date Collected	Quantity (µL)	Extracter
66	183	6/14/2018	300	Erin
67	184	6/14/2018	300	Erin
68	186	6/14/2018	300	Erin
69	215	6/14/2018	300	Erin
70	212	6/14/2018	300	Erin
71	121	6/15/2018	300	Erin
72	221	6/13/2018	300	Erin

Extraction Notes

• Digested for 3 hours and 40 minutes at 55°C
• Added additional 15µL Proteinase K after 2 hours and 50 min
• Some lipids left in samples
• Pelleted at 21130 rcf for 1 min

Qubit
Quantified May 22, 2019

• Followed Broad Range dsDNA and RNA Qubit protocol
• All samples were read twice

Extraction #	Sample	DNA Standard 1 (RFU)	DNA Standard 2 (RFU)	DNA 1 (ng/µl)	DNA 2 (ng/µl)	Average DNA	RNA Standard 1 (RFU)	RNA Standard 2 (RFU)	RNA 1 (ng/µl)	RNA 2 (ng/ul)	Average RNA
66	183	184.2	20357	2.80	2.80	2.80	385.6	10602	52.0	52.0	52.0
67	184	184.2	20357	2.20	2.14	2.17	385.6	10602	77.4	77.2	77.3
68	186	184.2	20357	6.12	6.00	6.06	385.6	10602	118	118	118
69	215	184.2	20357	13.3	13.2	13.25	385.6	10602	192	192	192
70	212	184.2	20357	7.86	7.90	7.88	385.6	10602	74.0	74.0	74.0
71	121	184.2	20357	23.4	23.0	23.2	385.6	10602	55.2	55.4	55.3
72	183	184.2	20357	Too Low	Too Low	Too Low	385.6	10602	43.8	43.2	43.5

Tape Station

• Followed RNA TapeStation protocol