Montipora Larvae DNA/RNA Extraction Batch 12

Larvae from Biomineralization Project


For detailed extraction protocol view post: Protocol for DNA/RNA Extractions of Montipora Coral Larvae Using Zymo-Duet Extraction Kit


Extraction Date: May 17, 2019

Samples

Extraction # Sample # Date Collected Quantity (µL) Extracter
66 183 6/14/2018 300 Erin
67 184 6/14/2018 300 Erin
68 186 6/14/2018 300 Erin
69 215 6/14/2018 300 Erin
70 212 6/14/2018 300 Erin
71 121 6/15/2018 300 Erin
72 221 6/13/2018 300 Erin

Extraction Notes

  • Digested for 3 hours and 40 minutes at 55°C
  • Added additional 15µL Proteinase K after 2 hours and 50 min
  • Some lipids left in samples
  • Pelleted at 21130 rcf for 1 min

Qubit
Quantified May 22, 2019

  • Followed Broad Range dsDNA and RNA Qubit protocol
  • All samples were read twice
Extraction # Sample DNA Standard 1 (RFU) DNA Standard 2 (RFU) DNA 1 (ng/µl) DNA 2 (ng/µl) Average DNA RNA Standard 1 (RFU) RNA Standard 2 (RFU) RNA 1 (ng/µl) RNA 2 (ng/ul) Average RNA
66 183 184.2 20357 2.80 2.80 2.80 385.6 10602 52.0 52.0 52.0
67 184 184.2 20357 2.20 2.14 2.17 385.6 10602 77.4 77.2 77.3
68 186 184.2 20357 6.12 6.00 6.06 385.6 10602 118 118 118
69 215 184.2 20357 13.3 13.2 13.25 385.6 10602 192 192 192
70 212 184.2 20357 7.86 7.90 7.88 385.6 10602 74.0 74.0 74.0
71 121 184.2 20357 23.4 23.0 23.2 385.6 10602 55.2 55.4 55.3
72 183 184.2 20357 Too Low Too Low Too Low 385.6 10602 43.8 43.2 43.5

Tape Station

TS-biomin-Ext-Batch-12-66.png
TS-biomin-Ext-Batch-12-67.png
TS-biomin-Ext-Batch-12-68.png
TS-biomin-Ext-Batch-12-69.png TS-biomin-Ext-Batch-12-70.png
TS-biomin-Ext-Batch-12-71.png TS-biomin-Ext-Batch-12-72.png

Written on May 22, 2019