Larvae from Biomineralization Project

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For detailed extraction protocol view post: Protocol for DNA/RNA Extractions of Montipora Coral Larvae Using Zymo-Duet Extraction Kit

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Extraction Date: March 18, 2019

Samples

Extraction #	Sample #	Date Collected	Quantity (µL)	Extracter
18	367	6/22/2018		Erin
19	321	6/20/2018	300	Erin
20	232	6/16/2018	300	Erin
21	322	6/20/2018	300	Erin

Extraction Notes

• Gathered all samples and set aside in cooler filled with dry ice. Then added DNA/RNA Shield to all.
• Digested for 3 hours at 55°C
• After digestion, pelleted out additional tissue in all samples. Centrifuged at 1600 rcf for 2 min.

Qubit
Quantified on March 21, 2019 with Batch 2

• Followed Broad Range dsDNA and RNA Qubit protocol
• All samples were read twice

Extraction #	Sample	DNA Standard 1 (RFU)	DNA Standard 2 (RFU)	DNA 1 (ng/µl)	DNA 2 (ng/µl)	Average DNA	RNA Standard 1 (RFU)	RNA Standard 2 (RFU)	RNA 1 (ng/µl)	RNA 2 (ng/ul)	Average RNA
18	367	192.3	19059	25.2	25.0	25.1	406.9	10912	30.0	30.6	30.3
19	321	192.3	19059	82.8	82.6	82.7	406.9	10912	73.2	73.8	73.5
20	232	192.3	19059	26.0	26.4	26.2	406.9	10912	43.4	43.2	43.3
21	322	192.3	19059	81.6	80.4	81.0	406.9	10912	74.6	75.2	74.9

Tape Station

• Followed RNA TapeStation protocol

Gel

• Followed PPP Lab Agarose Gel protocol
• Used the Thermo Scientific GeneRuler 1 kb Plus DNA Ladder (link)