Montipora Larvae DNA/RNA Extraction Batch 6
Larvae from Biomineralization Project
For detailed extraction protocol view post: Protocol for DNA/RNA Extractions of Montipora Coral Larvae Using Zymo-Duet Extraction Kit
Extraction Date: April 12, 2019
|Extraction #||Sample #||Date Collected||Quantity (µL)||Extracter|
- Gathered all samples and set aside on ice. Then added DNA/RNA Shield to all.
- Digested for 3 hours and 10 minutes at 55°C
- Some larvae did not appear to digest but liquid was dark yellow
- After digestion, pelleted out additional tissue in all samples. Centrifuged at 1600 rcf for 1 min.
- Added additional 15 µL of DNase I (without the buffer) to RNA sample 360.
Quantified on April 18, 2019
- Followed Broad Range dsDNA and RNA Qubit protocol
- All samples were read twice
|Extraction #||Sample||DNA Standard 1 (RFU)||DNA Standard 2 (RFU)||DNA 1 (ng/µl)||DNA 2 (ng/µl)||Average DNA||RNA Standard 1 (RFU)||RNA Standard 2 (RFU)||RNA 1 (ng/µl)||RNA 2 (ng/ul)||Average RNA|
|31||360||209.9||23014||Too Low||Too Low||Too Low||390.4||11319||Too Low||Too Low||Too Low|
|32||364||209.9||23014||Too Low||Too Low||Too Low||390.4||11319||Too Low||Too Low||Too Low|
|33||372||209.9||23014||2.20||2.16||2.18||390.4||11319||Too Low||Too Low||Too Low|