Larvae from Biomineralization Project

---

For detailed extraction protocol view post: Protocol for DNA/RNA Extractions of Montipora Coral Larvae Using Zymo-Duet Extraction Kit

---

Extraction Date: March 1, 2019

Samples

Extraction #	Sample #	Date Collected	Quantity (µL)	Extracter
10	218	6/15/2018	300	Erin
11	219	6/15/2018	300	Erin
12	220	6/15/2018	300	Erin
13	230	6/16/2018	300	Erin

Extraction Notes

• Digested for 3 hours and 20 minutes at 55°C
• Added 15 additional microliters of Protienase K after 2 hours of digestion at 55°C
• After digestion, pelleted out additional tissue in all samples

Qubit

• Followed Broad Range dsDNA and RNA Qubit protocol
• All samples were read twice

Extraction #	Sample	DNA Standard 1 (RFU)	DNA Standard 2 (RFU)	DNA 1 (ng/µl)	DNA 2 (ng/µl)	Average DNA	RNA Standard 1 (RFU)	RNA Standard 2 (RFU)	RNA 1 (ng/µl)	RNA 2 (ng/ul)	Average RNA
10	218	222.7	22939	23.8	25.2	24.5	413.8	11515	97.8	102.0	99.9
11	219	222.7	22939	29.0	30.0	29.5	413.8	11515	139.0	137.0	138.0
12	220	222.7	22939	16.4	16.3	16.35	413.8	11515	77.8	77.6	77.7
13	230	222.7	22939	24.4	23.8	24.1	413.8	11515	92.4	94.8	93.6

Tape Station

• Followed RNA TapeStation protocol